Predominance of HIV-1 among patients with AIDS and AIDS-related complex in Ghana
Identifieur interne : 001289 ( Main/Exploration ); précédent : 001288; suivant : 001290Predominance of HIV-1 among patients with AIDS and AIDS-related complex in Ghana
Auteurs : J. A. M. Brandful [Ghana] ; W. K. Ampofo [Ghana] ; F. A. Apeagyei [Ghana] ; K. Asare-Bediako [Ghana] ; M. Osei-Kwasi [Ghana]Source :
- East African medical journal [ 0012-835X ] ; 1997.
Descripteurs français
- KwdFr :
- Comorbidité, Dépistage systématique, Ghana (épidémiologie), Humains, Syndrome d'immunodéficience acquise (virologie), Syndrome d'immunodéficience acquise (épidémiologie), Syndrome pré-SIDA (virologie), Syndrome pré-SIDA (épidémiologie), Séroprévalence du VIH, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique), Études cas-témoins.
- MESH :
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- AIDS, AIDS-Related Complex (epidemiology), AIDS-Related Complex (virology), Acquired Immunodeficiency Syndrome (epidemiology), Acquired Immunodeficiency Syndrome (virology), Case-Control Studies, Comorbidity, Comparative study, Frequency, Ghana, Ghana (epidemiology), HIV Seroprevalence, HIV-1 (genetics), HIV-1 virus, HIV-2 (genetics), HIV-2 virus, Human, Humans, Mass Screening, Prevalence.
- MESH :
- geographic , epidemiology : Ghana.
- epidemiology : AIDS-Related Complex, Acquired Immunodeficiency Syndrome.
- genetics : HIV-1, HIV-2.
- virology : AIDS-Related Complex, Acquired Immunodeficiency Syndrome.
- Case-Control Studies, Comorbidity, HIV Seroprevalence, Humans, Mass Screening.
Abstract
We determined the prevalence of HIV among AIDS and AIDS-Related Complex (ARC) patients seen within one year in two hospitals in southern Ghana. Subjects were screened by an ELISA procedure for anti-HIV antibodies. Specific identification of the HIV type was done with a particle agglutination (PA) kit. All PA-determined dual specimens were then confirmed by Western blotting and Pepti-Lav 1/2 monoepitope kit. Virus isolation was attempted from symptomatic patients by co-culturing patient peripheral blood monocyte cells (PBMCs) and CD4+ cell lines. PBMCs and HIV isolates were characterised by PCR. By ELISA, 43.5% of the subjects (253) had anti-HIV antibodies. Of these, 61(24%) were HIV-1 positive and 42 (18.6%) were dually reactive by PA. However, only 19% were confirmed as true dually-infected cases by western blotting and Pepti-Lav though all 42 samples were HIV-1 positive on the two tests. No subject was infected with HIV-2 alone. Three viruses were isolated. By PCR two of them had both HIV-1 and HIV-2 proviral sequences while the third virus was HIV-1 only. HIV-1 prevalence now predominates over HIV-2 implying a switch in the HIV infection pattern in Ghana. Furthermore mixed infections exist. The predominance of HIV-1 infection in Ghana may indicate a similar trend in other parts of West Africa.
Affiliations:
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A. M." last="Brandful">J. A. M. Brandful</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Ampofo, W K" sort="Ampofo, W K" uniqKey="Ampofo W" first="W. K." last="Ampofo">W. K. Ampofo</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Apeagyei, F A" sort="Apeagyei, F A" uniqKey="Apeagyei F" first="F. A." last="Apeagyei">F. A. 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Osei-Kwasi</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Virology Unit, Noguchi Memorial Institute for Medical Research (NMIMR), University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author></analytic><series><title level="j" type="main">East African medical journal</title><title level="j" type="abbreviated">East Afr. med. j.</title><idno type="ISSN">0012-835X</idno><imprint><date when="1997">1997</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">East African medical journal</title><title level="j" type="abbreviated">East Afr. med. j.</title><idno type="ISSN">0012-835X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>AIDS</term><term>AIDS-Related Complex (epidemiology)</term><term>AIDS-Related Complex (virology)</term><term>Acquired Immunodeficiency Syndrome (epidemiology)</term><term>Acquired Immunodeficiency Syndrome (virology)</term><term>Case-Control Studies</term><term>Comorbidity</term><term>Comparative study</term><term>Frequency</term><term>Ghana</term><term>Ghana (epidemiology)</term><term>HIV Seroprevalence</term><term>HIV-1 (genetics)</term><term>HIV-1 virus</term><term>HIV-2 (genetics)</term><term>HIV-2 virus</term><term>Human</term><term>Humans</term><term>Mass Screening</term><term>Prevalence</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Comorbidité</term><term>Dépistage systématique</term><term>Ghana (épidémiologie)</term><term>Humains</term><term>Syndrome d'immunodéficience acquise (virologie)</term><term>Syndrome d'immunodéficience acquise (épidémiologie)</term><term>Syndrome pré-SIDA (virologie)</term><term>Syndrome pré-SIDA (épidémiologie)</term><term>Séroprévalence du VIH</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2) (génétique)</term><term>Études cas-témoins</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Ghana</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>AIDS-Related Complex</term><term>Acquired Immunodeficiency Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term><term>HIV-2</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>VIH-2 (Virus de l'Immunodéficience Humaine de type 2)</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome d'immunodéficience acquise</term><term>Syndrome pré-SIDA</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>AIDS-Related Complex</term><term>Acquired Immunodeficiency Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Ghana</term><term>Syndrome d'immunodéficience acquise</term><term>Syndrome pré-SIDA</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Case-Control Studies</term><term>Comorbidity</term><term>HIV Seroprevalence</term><term>Humans</term><term>Mass Screening</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Comorbidité</term><term>Dépistage systématique</term><term>Humains</term><term>SIDA</term><term>Prévalence</term><term>Séroprévalence du VIH</term><term>Virus HIV1</term><term>Fréquence</term><term>Virus HIV2</term><term>Etude comparative</term><term>Ghana</term><term>Homme</term><term>Études cas-témoins</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We determined the prevalence of HIV among AIDS and AIDS-Related Complex (ARC) patients seen within one year in two hospitals in southern Ghana. Subjects were screened by an ELISA procedure for anti-HIV antibodies. Specific identification of the HIV type was done with a particle agglutination (PA) kit. All PA-determined dual specimens were then confirmed by Western blotting and Pepti-Lav 1/2 monoepitope kit. Virus isolation was attempted from symptomatic patients by co-culturing patient peripheral blood monocyte cells (PBMCs) and CD4+ cell lines. PBMCs and HIV isolates were characterised by PCR. By ELISA, 43.5% of the subjects (253) had anti-HIV antibodies. Of these, 61(24%) were HIV-1 positive and 42 (18.6%) were dually reactive by PA. However, only 19% were confirmed as true dually-infected cases by western blotting and Pepti-Lav though all 42 samples were HIV-1 positive on the two tests. No subject was infected with HIV-2 alone. Three viruses were isolated. By PCR two of them had both HIV-1 and HIV-2 proviral sequences while the third virus was HIV-1 only. HIV-1 prevalence now predominates over HIV-2 implying a switch in the HIV infection pattern in Ghana. Furthermore mixed infections exist. The predominance of HIV-1 infection in Ghana may indicate a similar trend in other parts of West Africa.</div></front></TEI><affiliations><list><country><li>Ghana</li></country><region><li>Région du Grand Accra</li></region><settlement><li>Accra</li><li>Legon (Ghana)</li></settlement><orgName><li>Université du Ghana</li></orgName></list><tree><country name="Ghana"><region name="Région du Grand Accra"><name sortKey="Brandful, J A M" sort="Brandful, J A M" uniqKey="Brandful J" first="J. A. M." last="Brandful">J. A. M. Brandful</name></region><name sortKey="Ampofo, W K" sort="Ampofo, W K" uniqKey="Ampofo W" first="W. K." last="Ampofo">W. K. Ampofo</name><name sortKey="Apeagyei, F A" sort="Apeagyei, F A" uniqKey="Apeagyei F" first="F. A." last="Apeagyei">F. A. Apeagyei</name><name sortKey="Asare Bediako, K" sort="Asare Bediako, K" uniqKey="Asare Bediako K" first="K." last="Asare-Bediako">K. Asare-Bediako</name><name sortKey="Osei Kwasi, M" sort="Osei Kwasi, M" uniqKey="Osei Kwasi M" first="M." last="Osei-Kwasi">M. Osei-Kwasi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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